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Haploinsufficiency of the TDP43 ubiquitin E3 ligase RNF220 leads to ALS-like motor neuron defects in the mouse
Pengcheng Ma1,† , Yuwei Li1,2,† , Huishan Wang1,2 , Bingyu Mao1,3,*
1State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
2Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming 650203, China
3Center for Excellence in Animal Evolution and Genetics, Chinese Academy of Sciences, Kunming 650223, China
These authors contributed equally to this work.
*Correspondence to:Bingyu Mao ,
J Mol Cell Biol, Volume 13, Issue 5, May 2021, Pages 374-382
Keyword: TDP43, RNF220, amyotrophic lateral sclerosis (ALS), polyubiquitination

TDP43 pathology is seen in a large majority of amyotrophic lateral sclerosis (ALS) cases, suggesting a central pathogenic role of this regulatory protein. Clarifying the molecular mechanism controlling TDP43 stability and subcellular location might provide important insights into ALS therapy. The ubiquitin E3 ligase RNF220 is involved in different neural developmental processes through various molecular targets in the mouse. Here, we report that the RNF220+/− mice showed progressively decreasing mobility to different extents, some of which developed typical ALS pathological characteristics in spinal motor neurons, including TDP43 cytoplasmic accumulation, atrocytosis, muscle denervation, and atrophy. Mechanistically, RNF220 interacts with TDP43 in vitro and in vivo and promotes its polyubiquitination and proteasomal degradation. In conclusion, we propose that RNF220 might be a modifier of TDP43 function in vivo and contribute to TDP43 pathology in neurodegenerative disease like ALS.