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OIP5-AS1 specifies p53-driven POX transcription regulated by TRPC6 in glioma
Wei Shao1,† , Zhen-Yu Hao1,† , Yi-Fei Chen1,† , Jun Du1 , Qian He1 , Liang-Liang Ren1 , Yan Gao1 , Nan Song1 , Yan Song1 , Hua He2 , Yi-Zheng Wang1,*
1The Brain Science Center, Beijing Institute of Basic Medical Sciences, Beijing 100850, China
2Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
These authors contributed equally to this work.
*Correspondence to:Yi-Zheng Wang ,
J Mol Cell Biol, Volume 13, Issue 6, June 2021, Pages 409-421
Keyword: DNA‒RNA hybrid, glioma, lncRNA, p53, transcription

Transcription factors (TFs) control an array of expressed genes. However, the specifics of how a gene is expressed in time and space as controlled by a TF remain largely unknown. Here, in TRPC6-regulated proline oxidase 1 (POX) transcription in human glioma, we report that OIP5-AS1, a long noncoding RNA, determines the specificity of p53-driven POX expression. The OIP5-AS1/p53 complex via its 24 nucleotides binds to the POX promoter and is necessary for POX expression but not for p21 transcription. An O-site in the POX promoter to which OIP5-AS1 binds was identified that is required for OIP5-AS1/p53 binding and POX transcription. Blocking OIP5-AS1 binding to the O-site inhibits POX transcription and promotes glioma development. Thus, the OIP5-AS1/O-site module decides p53-controlled POX expression as regulated by TRPC6 and affects glioma development.