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Measurement of dynamic membrane mechanosensation using optical tweezers
Xuanling Li1 , Xing Liu2 , Xiaoyu Song2 , Yinmei Li1,2 , Ming Li3 , Haowei Wang1,2,*
1Department of Optics and Optical Engineering, University of Science and Technology of China and Hefei National Laboratory for Physical Sciences at the Microscale, Hefei, China
2MOE Key Laboratory for Membraneless Organelles & Cellular Dynamics, Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, Hefei, China
3Department of Laboratory Diagnostics, the First Affiliated Hospital, University of Science and Technology of China, Hefei, China
*Correspondence to:Haowei Wang ,
J Mol Cell Biol, Volume 13, Issue 6, June 2021, Pages 455-457

Many cellular processes are orchestrated by dynamic changes in the plasma membrane to form membrane projections and endocytic compartments in response to extracellular cue changes. Our previous studies show that post-translational modifications of ACAP4 regulate membrane dynamics and curvature in response to epidermal growth factor and chemokine (C−C motif) ligand 18 stimulation (Zhao et al., 2013; Song et al., 2018). However, there is no quantitative measurement to annotate magnitude of dynamic membrane cytoskeletal remodeling in stimulus-elicited mechanosensation on the plasma membrane.