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Enhancing prime editing efficiency by modified pegRNA with RNA G-quadruplexes
Xiangyang Li1,2,† , Xin Wang1,2,† , Wenjun Sun1,2,† , Shisheng Huang1,2 , Mingtian Zhong3 , Yuan Yao4 , Quanjiang Ji5 , Xingxu Huang1,6,*
1Gene Editing Center, School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China
2University of Chinese Academy of Sciences, Beijing 100049, China
3Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, Center for Studies of Psychological Application, South China Normal University, Guangzhou 510631, China
4Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou 311215, China
5School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China
6Guangzhou Laboratory, Guangzhou International Bio Island, Guangzhou 510005, China
These authors contributed equally to this work.
*Correspondence to:Xingxu Huang , Email:huangxx@shanghaitech.edu.cn
J Mol Cell Biol, Volume 14, Issue 4, April 2022, mjac022,  https://doi.org/10.1093/jmcb/mjac022

Dear Editor,

Recent study shows that the prime editing system fusing the Cas9 nickase and reverse transcriptase could perform all types of gene modifications, including base substitutions (transitions and transversions), small insertions, and deletions, without requiring donor DNA or double-strand breaks (DSBs) (Anzalone et al., 2019). Despite the accuracy and versatility, the efficiency of the prime editor (PE) is often insufficient, which limits its broad applications.