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The regenerative response of cardiac interstitial cells
Laura Rolland1,† , Alenca Harrington1,† , Adèle Faucherre1 , Jourdano Mancilla Abaroa1 , Girisaran Gangatharan1 , Laurent Gamba1 , Dany Severac1,2 , Marine Pratlong1,2 , Thomas Moore-Morris1,* , Chris Jopling1,*
1Institute of Functional Genomics, University of Montpellier, CNRS, INSERM, LabEx ICST, Montpellier 34094, France
2Montpellier GenomiX, France Génomique, Montpellier 34094, France
These authors contributed equally to this work
*Correspondence to:Thomas Moore-Morris , Chris Jopling ,
J Mol Cell Biol, Volume 14, Issue 10, October 2022, mjac059,
Keyword: heart regeneration, interstitial cells, single-cell RNA-sequencing, macrophage, fibroblast, endothelium, inflammation

Understanding how certain animals are capable of regenerating their hearts will provide much needed insights into how this process can be induced in humans in order to reverse the damage caused by myocardial infarction. Currently, it is becoming increasingly evident that cardiac interstitial cells play crucial roles during cardiac regeneration. To understand how interstitial cells behave during this process, we performed single-cell RNA sequencing of regenerating zebrafish hearts. Using a combination of immunohistochemistry, chemical inhibition, and novel transgenic animals, we were able to investigate the role of cell type-specific mechanisms during cardiac regeneration. This approach allowed us to identify a number of important regenerative processes within the interstitial cell populations. Here, we provide detailed insight into how interstitial cells behave during cardiac regeneration, which will serve to increase our understanding of how this process could eventually be induced in humans.