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SNAP23–Kif5 complex controls mGlu1 receptor trafficking Free
Fabrice Raynaud 1,3 , Vincent Homburger 1 , Martial Seveno 2 , Oana Vigy 1 , Enora Moutin 1 , Laurent Fagni 1 , and Julie Perroy 1,*
1 IGF, CNRS, INSERM, Univ. Montpellier, F-34094 Montpellier, France
2 BioCampus Montpellier, CNRS, INSERM, Univ. Montpellier, F-34094 Montpellier, France
3 Present address: INSERM U1046 PhyMedExp, CNRS UMR9214, Univ. Montpellier, F-34094 Montpellier, France
*Correspondence to:Julie Perroy, E-mail: Julie.Perroy@igf.cnrs.fr
J Mol Cell Biol, Volume 10, Issue 5, October 2018, Pages 423-436  https://doi.org/10.1093/jmcb/mjy031
Keyword: mGlu1, SNARE, kinesin, neuron, traffic

Metabotropic glutamate receptors are expressed at excitatory synapses and control synaptic transmission in mammalian brain. These receptors are involved in numerous patho-physiological functions. However, little is known about the molecular determinants responsible for their intracellular transport and membrane targeting. Here we investigated the nature of the molecular motor and adaptor protein responsible for trafficking and membrane localization of the group I metabotropic glutamate mGlu1 postsynaptic receptor in cultured hippocampal neurons. In proteomic studies, we identified the synaptosome-associated protein 23 (SNAP23) and the molecular motor Kif5 kinesin as proteins interacting with mGlu1 receptor. We showed that SNAP23, but not Kif5, directly interacts with mGlu1 receptor carboxyl terminus. Using a recombination approach to impair or enhance the interaction between SNAP23 and Kif5, we found that the SNAP23–Kif5 complex controls the trafficking of mGlu1 receptor along microtubules. Additional fluorescence recovery after cleavage experiments allowed us to identify a role of the complex in the receptor cell surface targeting. In conclusion, our study indicates that along dendritic processes Kif5–SNAP23 complex contributes to proper mGlu1 receptor trafficking and cell surface expression.