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Trafficking pathway between plasma membrane and mitochondria via clathrin-mediated endocytosis Free
Zhongya Wei 1,2 , Wenfeng Su 2 , Huifang Lou 1 , Shumin Duan 1,* , and Gang Chen 2,3,*
1 Department of Neurobiology, Key Laboratory of Medical Neurobiology of Ministry of Health of China, Key Laboratory of Neurobiology of Zhejiang Province,Zhejiang University School of Medicine, Hangzhou 310058, China
2 Key Laboratory of Neuroregeneration of Jiangsu Province and the Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226019, China
3 Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong 226001, China
*Correspondence to:* Correspondence to: Shumin Duan, E-mail: duanshumin@zju.edu.cn; Gang Chen, E-mail: chengang6626@ntu.edu.cn
J Mol Cell Biol, Volume 10, Issue 6, December 2018, Pages 539-548  https://doi.org/10.1093/jmcb/mjy060
Keyword: endocytosis, mitochondria, FM dye, clathrin

Endocytosis is a basic cellular process that describes a form of active transport across the plasma membrane into the cell. The endocytic pathway consists of distinct membrane compartments; internalized molecules are delivered to early endosomes, and some of them are recycled back to the surface, whereas other molecules are sent to late endosomes and lysosomes for degradation. However, little is known about how mitochondria are involved in the endocytic pathway. Here, we report that FM dyes, membrane-impermeant fluorescent lipid probes, can traffic to mitochondria directly from the plasma membrane by clathrin-mediated endocytosis. FM dye entry into mitochondria uses microtubule-dependent active transport, but the mechanism is different from the classical endocytic pathway. Hence, this study reveals a previously unrealized lipid trafficking pathway from the plasma membrane to mitochondria.